FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471239
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG180050.5186908766581616No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC102780.29609024328200967No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA99900.28779349390808295No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC96880.2790934303284792No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87880.25316608853495826No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT54030.15565047523377099No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47230.1360609281008885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG35900.062.7910354
TACGGCC36450.061.7506187
TCTACGG36600.061.5901155
CTACGGC37800.059.725376
ACGGCCA39400.057.30088
CGGCCAT40500.055.660029
GCCATAC40750.055.0555811
GGGTCTA29850.054.3152662
CCTGGTT63600.052.39020570
GCGCGGT55050.052.3200049
CCATACC43700.051.5052112
CATACCA43700.051.5052113
GCGTCTA7050.051.4285932
GGCCATA43850.051.32976510
CCGAACT9550.050.77999567
TACCACC45300.049.68388715
GCCGGTT9400.049.4878923
TGGCGCG59350.048.52653515
TGAACGC46500.048.46751423
CGGTTCA9500.048.248955