FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458059
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG180370.5215931827652449No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC102800.2972765936035215No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA98840.28582508279933916No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC97190.2810536199642632No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85930.24849200085944168No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT53460.15459539585646168No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG46900.13562521634246263No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34750.10048989910235771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG37000.063.900124
TACGGCC37300.063.2926147
TCTACGG37450.063.040935
CTACGGC38350.061.5605966
ACGGCCA41100.058.0226338
GGGTCTA31250.056.2893942
CGGCCAT42250.055.957449
GCCATAC43050.054.7586311
CCATACC44100.053.6100212
CCTGGTT63000.053.43535270
GCGTCTA7500.053.3873142
CATACCA44900.052.42622413
GCGCGGT50650.051.60849
CGTCTAC8700.051.5201873
GGCCATA46500.051.21094510
TACCACC47850.049.337115
TGGCGCG52850.049.26587315
GAACGCG49000.048.051724
CTGAACG49400.047.6259222
CCCGATC49550.047.3138931