FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460713
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG175370.5067452863037183No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC120000.34674935482948166No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG114030.32949857442671493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87730.2535026741599202No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC87200.2519711978427567No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG48580.14037569714680184No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG46320.13384525096417993No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45600.13176475483520303No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA40960.11835711311512975No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40930.11827042577642237No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT40880.11812594687857676No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG37890.10948610878740883No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG35640.10298455838435605No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT34840.10067289601882617No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA34630.10006608464787459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18550.041.5267470
AGGACCG9150.037.2913770
CGCGGCA51800.036.9645532
GCGGCAC52400.036.56960333
GTTCCGT3700.036.183841
CGGCACC53950.035.85990534
TCGCCGG53450.034.99912648
CCGTCGC54400.034.26595345
CCCGTCG55900.033.8165644
CCCCCGA60850.031.7979819
GCACCCC62000.031.46498536
CACCCCC62850.031.23519337
GGCACCC62850.031.02490435
CCCCCGT60350.031.01768142
ACCCGCG62300.030.91960725
CCCCGAC63600.030.43497720
ATGGCCG20600.030.18838169
GATAGGC33600.029.9691740
AGTCCGC67000.029.1984797
ATAGGCA36000.028.29510741