FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471024
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG175160.5046349434633699No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC119130.3432128386320578No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG112370.32373731786354687No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC88370.2545934571469399No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86940.2504736354459087No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG47430.13664555474119452No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47330.136357455321542No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG46370.13359170089287772No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40970.11803433223164116No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA40800.11754456321823184No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT40740.11737170356644035No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG37270.10737465370449757No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG35990.10368698113294521No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT34890.10051788751676738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19250.049.40609470
ATGGCCG21000.034.97958469
CGCGGCA52750.034.2080632
AGGACCG8100.033.36403370
GCGGCAC54800.033.32507733
CGGCACC55500.032.6194334
TCGCCGG54700.032.13020348
CCGTCGC54250.032.08312245
CCCGTCG56000.031.29740344
GACGTAT2950.030.70998844
CCCCCGA60350.030.2506419
ACCCGCG60150.030.20338825
CGTACGT1600.029.97041519
TATCGGA3050.029.1603878
GCACCCC63400.028.7032136
TTATCGG3100.028.6900587
CCCCGAC63900.028.4768520
GTTATCG3150.028.234666
CACCCCC64550.028.16252337
GGCACCC64400.028.12623635
Produced by FastQC (version 0.11.5)