FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152233
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG157700.5002802775048671No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC98510.31250862483832886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97700.30993901783275535No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG91590.2905559328894787No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC72590.2302812006599766No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG46080.14618208742818187No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40550.12863896799506888No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG40300.12784587941310177No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT35870.11379234974064417No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.10763798234457922No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA31540.10005605550097343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT13100.038.90976770
CGCGGCA46950.037.61158832
GCGGCAC47700.037.03805533
CCCGTCG47400.036.12121244
TCGCCGG47300.036.04871448
CCGTCGC47600.035.92645645
CGGCACC50100.035.42683434
ACCCGCG53750.033.02995725
CCCCCGA54050.032.8693719
CACCCCC57150.031.55493737
CCCCCGT54100.031.53479442
GCACCCC57900.031.47463836
CCCCGAC56400.031.39870620
ATGGCCG13300.030.43827269
GGCACCC58850.030.293635
GCGCGGG59250.029.75429562
CCGCGCG60350.029.62476227
AGTCCGC62850.028.6135547
GATAGGC32000.028.5680740
CAGTCCG60600.028.48896