FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152233
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG108770.34505698024226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96480.30606874555275576No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC94510.29981920752685476No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89940.2853215482484956No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA89570.28414777714718426No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44030.1396787610560514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG22200.060.630994
TCTACGG22950.058.4994165
TACGGCC23100.058.4170077
CTACGGC23850.056.5790946
ACGGCCA26000.052.1647228
CCGAACT8550.050.9512467
GCCATAC26500.050.65383511
GCGCGGT45550.050.528459
CGGCCAT26900.050.037479
GGGTCTA20850.049.1117742
CCATACC28150.047.81175212
GGCCATA28500.047.3485210
CGGTCTA4450.046.9451072
TGGCGCG49000.046.8265715
CATACCA28750.046.69482413
CGCGGTG50700.045.6660910
TACCACC29850.044.97336215
GCCGGTT9450.044.2128523
CGGTTCA9600.043.881565
GAACGCG31250.043.01465624
Produced by FastQC (version 0.11.5)