FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130073
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG112340.3589053673828055No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC97460.31136654001360353No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94470.30181404714842114No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89680.2865108896821256No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA88490.2827090614180564No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44520.1422331044675316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG22350.060.380584
TCTACGG22500.059.9780465
TACGGCC22750.059.0159457
CTACGGC23650.056.7682766
GCCATAC25200.053.68516511
ACGGCCA25600.052.980978
GCGCGGT47600.051.231589
CGGCCAT26800.050.225299
GGGTCTA21750.049.9204672
CCATACC27500.049.19513712
GCGTCTA4700.048.8264922
CATACCA27950.048.4030813
GGCCATA28200.047.8532910
CCGAACT7800.047.09596667
TGGCGCG52400.046.79781315
CGCGGTG52450.046.7554510
TACCACC29900.045.01582315
CGGTTCA8250.044.8382235
CTGAACG29700.044.75683222
GCCGGTT8450.044.5869373
Produced by FastQC (version 0.11.5)