FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120961
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG155490.4982119289539344No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC98120.314390343230819No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96610.3095520898851348No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG90080.28862904727101685No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC71920.23044184147126479No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45780.14668558818902255No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39820.12758890610936824No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG39220.12566642133624867No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT36450.11679094996701336No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.11518887932274707No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA31640.10137903036917155No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT31410.10064207787280906No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG31210.10000124961510252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA45650.039.07805332
GCGGCAC47950.037.07443633
TCGCCGG47150.036.7351848
CCCGTCG47950.036.06153544
CCGTCGC47750.036.02358245
CGGCACC49350.035.84091634
TGGCCGT13950.034.72302270
ACCCGCG52650.033.66672525
CCCCCGA55000.032.24981319
GCACCCC55600.032.10743736
CCCCCGT54150.031.75815642
CCCCGAC56750.031.21043420
CACCCCC57600.030.89533837
GGCACCC58500.030.54673635
TTATCGG2500.030.1520187
CGCCCTA11200.030.1515361
GTGCCGT6050.029.6680831
GTTATCG2550.029.5608026
CCGCGCG60000.029.52473827
AGTCCGC61800.029.1629227