FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336015
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA209370.6276050917037244No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC100840.3022768182996779No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC88860.26636570878728066No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57440.17218147999934055No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA52250.15662399599522184No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51700.15497532235316688No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48030.1439741727780001No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC45260.13567085279892327No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA44000.13189389136439733No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG42770.1282068575830744No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC38750.11615655205387265No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT35050.10506547482550287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA8650.054.0817535
CCGAACT8500.054.07939567
GGGGACT40150.054.0100331
GCGCGGT48100.052.56189
ACTCTTC44750.051.9614945
GCCGGTT9000.051.6002543
GGACTCT49050.050.0748633
CGCGGTG50850.049.9236610
CGGGACT10150.049.6692051
TCTGGTC46150.049.5668110
TGGCGCG52200.049.02865215
GGGACTC50650.048.4294782
TTCTGGT47250.048.4114159
CCGGTTC9750.048.333024
TCTTCTG49450.046.882967
CTCTTCT49850.046.7834786
GTTGATC8600.046.39813610
GCCGAAC9850.046.1005866
CTTAGGC9900.045.8868718
TCCTTAG9850.045.74841716
Produced by FastQC (version 0.11.5)