FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328750
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA210970.6337814494930529No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103670.31143822756289896No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89090.26763800225309803No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA56910.17096507698084867No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55130.16561772437101013No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT52340.1572361997746902No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC49070.14741269245212166No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC48130.14458880961321818No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG47330.14218550506947053No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA45140.13560645888096132No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC41680.12521216672925273No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT36850.11070221554637628No Hit
GGGGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACG33740.10135936913255726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT41100.054.88981
GCGCGGT49950.052.126659
ACTCTTC47500.051.411965
CCGAACT8850.050.8028567
CGGTTCA8550.050.6878475
GCCGGTT8700.049.813923
CGCGGTG52550.049.47767610
GGACTCT52200.049.2208983
TCTGGTC50000.048.83695610
TGGCGCG53750.048.18327715
TTCTGGT51200.047.8309979
CCGGTTC9100.047.62434
TCTTCTG52650.046.9710127
GGGACTC53100.046.9630132
TGCGTTA1100.046.900615
CTTAGGC9100.046.8923418
CGGGTAG3400.046.6696621
CTCTTCT53300.046.20466
GGTACGA2100.045.8604353
GCCGAAC9850.045.7970866
Produced by FastQC (version 0.11.5)