FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376244
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA219540.6502492118460632No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103460.3064351984039068No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC87800.26005229479859865No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56660.1678196244110319No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA55120.16325834270271936No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51990.15398768572413604No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48310.14308799956401255No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG46540.13784548747069228No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC46170.1367495951122016No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA44760.13257335666498038No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC39930.11826751857981828No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT36080.1068643143090369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA353.3542165E-970.9118260
GGGGACT40250.055.4210781
CCGAACT7950.054.27980867
GCGCGGT46900.052.800429
CGGTTCA8100.052.2281045
ACTCTTC48100.051.9844555
CGGGACT10500.051.6319731
GGACTCT52950.049.624793
CGCGGTG50050.049.408610
GGGACTC51200.049.3729022
TCTGGTC49800.049.31131710
GCCGGTT8650.049.30343
TTCTGGT51150.048.547779
TGGCGCG51100.048.12341315
CTTAGGC8900.047.9440818
TGCGTTA1450.047.43875515
CCGGTTC9000.047.00394
TTAGGCA9200.046.76722319
GCCGAAC9200.046.70699366
TCTTCTG54000.046.6875237
Produced by FastQC (version 0.11.5)