FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776461
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG198560.5257832663967666No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC138070.36560684725726017No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA129790.34368155794538857No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC123940.3281908644098271No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT62000.16417487165894207No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG55210.14619507523048694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG40200.063.6658864
TCTACGG41200.062.1222425
TACGGCC41750.060.890217
CTACGGC42500.059.734416
CGGCCAT45000.056.4165769
GCCATAC45100.056.1383111
ACGGCCA45500.055.8717848
GGGTCTA35050.054.5929262
CCATACC46600.054.55292512
GCGCGGT64850.054.008229
GCGTCTA7200.053.7279362
CCTGGTT67700.053.08842570
CATACCA48950.052.14562613
GGCCATA49000.051.88163410
TACCACC49500.051.6360115
CCGAACT9200.051.00232767
TGGCGCG69000.050.50893415
CGCGGTG69600.050.4215710
CTGAACG51900.049.22929422
CGTCTAC9450.048.6109923
Produced by FastQC (version 0.11.5)