FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4079292
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122940.3013758269817409No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC107450.26340355140058613No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA105620.2589174788174026No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC90790.22256313105313372No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG83940.20577100143848492No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45930.11259306762055768No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC41610.10200299463730472No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41520.10178236811682027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG17750.056.66754
TACGGCC18450.054.3281027
TCTACGG18600.054.077855
CTACGGC19450.051.7139136
GCGCGGT54950.051.0366029
TGGCGCG58350.047.8274315
GCCATAC21500.046.61994611
CGCGGTG62250.045.27215610
CCGAACT9050.045.04701667
CGGCCAT22500.045.005639
CATACCA23050.043.93174713
GAACGCG22350.043.47029524
ACGGCCA23300.043.165738
ATCTCGT22500.043.0065635
GGGTCTA18750.042.303562
CTACTCG68200.041.8054737
TGAACGC23400.041.79612723
CCATACC23850.041.73851412
CGGTTCA9550.041.69845
TACTCGG68550.041.6670338