FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089962
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG300450.7346034021831988No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC159560.39012587403012544No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124380.3041104049377476No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC115380.28210531051388743No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG90930.22232480399573396No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG87200.21320491486228965No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG82280.20117546324391278No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA71670.1752339019286732No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC57260.14000130074558148No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT56420.13794749193268788No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.12831415059602022No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG50870.12437768370464077No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49010.11982996419037634No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG47950.11723825306934393No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA47420.11594239750882772No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG46090.11269053355507949No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT42780.10459754882808203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA90150.036.60503432
GCGGCAC92750.035.74946233
CGGCACC93650.035.4238734
CCGTCGC88900.034.136645
CCCGTCG89550.034.06936344
TCGCCGG94400.033.26442748
GCACCCC100950.033.039736
GGCACCC102650.032.60917735
CCCCCGA103200.032.41082419
ACCCGCG102750.032.30783525
TAGTCCG5350.032.085086
CACCCCC103550.032.06438437
TGGCCGT14900.030.42881670
CCCCGAC109950.030.4017520
GTCCGTT4000.029.1838842
TTATCGG2950.029.0940977
TATCGGA2850.028.9103478
GTTCCGT3000.028.7119731
CCGCGCG115050.028.67901227
TCAGGAT26900.028.65285970