FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089962
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123800.30269229885265436No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC106680.26083371923748927No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA103540.2531563862940536No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC92570.22633462120185957No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG86970.2126425624492355No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47770.11679815118086671No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42630.10423079725435103No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41810.1022258886512882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG19100.057.514614
TCTACGG19700.055.5886345
TACGGCC20050.055.1312377
CTACGGC20950.052.7608956
GCGCGGT56950.051.8395239
CCGAACT9600.050.68513567
GCCATAC22650.048.04490311
ACGGCCA23700.046.9302568
TGGCGCG63050.046.5501815
CGCGGTG63900.046.14754510
CGGCCAT24150.045.7714849
CGGTTCA10300.044.6610765
CCATACC24400.044.5990612
CATACCA25200.044.00056513
GGGTCTA19650.043.6756172
CCCGATC24850.043.58670431
ATCTCGT24700.042.82957535
GCCGGTT10900.042.517613
GAACGCG25500.042.4226524
CGGCATA2950.042.161831