FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086457
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG297600.7282592230873834No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC161500.3952078781203375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124250.30405311985419153No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC118360.2896396560639204No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG89470.21894271737106252No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG88390.2162998411582454No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG83440.20418665851616694No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA69670.17048998680274868No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT58170.1423482493514553No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC56100.1372827366102225No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52520.12852209138625464No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG50510.12360340510128946No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49330.1207158181280263No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG48970.11983485939042061No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG46490.11376603253135907No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA46450.11366814822718066No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT42070.10294981691964457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA90000.036.40945432
GCGGCAC91850.035.65782533
CGGCACC93450.035.10788734
CCGTCGC88550.033.6951245
CCCGTCG89200.033.62568744
GCACCCC98350.033.4437636
TCGCCGG92900.033.3548748
CCCCCGA99250.033.04652419
ACCCGCG100100.032.72062325
CACCCCC101150.032.46946737
GGCACCC101650.032.29539535
TAGTCCG4150.030.6048016
CCCCGAC107100.030.57480420
GATAGGC44800.029.53528640
ATAGGCA46350.028.94483441
CATTAGC46100.028.74164613
CTGATAG46700.028.52146138
CCGCGCG116100.028.39094527
GCATTAG46650.028.18272812
CCCGCGC117050.028.03011726