FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086457
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122990.30096976427257155No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC101210.24767176064742638No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA97630.2389111154234585No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC86410.21145456810141403No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG82650.2022534435086433No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44010.10769720567229756No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41620.10184861849763743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG17000.056.9448284
TACGGCC17900.053.895197
TCTACGG18300.053.0923655
CTACGGC18900.051.5885476
GCGCGGT54850.049.009689
TGGCGCG59750.044.81314515
GCCATAC21100.044.7452511
ACGGCCA22100.043.9633648
CGGCCAT22200.043.610689
CGCGGTG62800.043.024110
CCGAACT8850.042.79641767
CGGTTCA9000.042.7240375
GCGTCTA4700.041.6400342
CGGGTAG5550.041.1580471
GCCGGTT9550.040.977453
CATACCA23300.040.95940413
GTAGTCC65600.040.87667527
CTACTCG65950.040.46347437
CGTGCCT66150.040.39768620
GGGTCTA18650.040.3180352