FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3620633
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA175510.48474948993725686No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG131820.36407998269915787No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC127010.3507950129162497No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC123080.33994055735557843No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT57680.15930915947570495No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA46370.12807152782400205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC27200.061.4268537
GTCTACG27400.060.6030854
TCTACGG27600.060.539965
CTACGGC28600.058.301416
GCCATAC30550.054.5755211
GCGCGGT60600.054.0546959
CGGCCAT31200.053.5486539
ACGGCCA31300.053.4880878
CCATACC31750.052.6210412
CGCGGTG63900.051.26313410
GGCCATA32650.051.1705410
CCTGGTT47000.050.76624370
CATACCA33200.050.63679513
TGGCGCG64750.050.43131315
GGGTCTA25850.049.5310252
TGAACGC33800.049.26638423
GAACGCG33800.049.0696824
ATCTCGT34200.048.5905435
CTGAACG34350.048.57228522
GCCCGAT34700.047.4378730
Produced by FastQC (version 0.11.5)