FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625654
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG170100.4691567369638691No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC151300.4173040229431711No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG121990.33646343528643385No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC114700.3163567179879823No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT65070.17947106921951184No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61220.16885229533761356No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA60810.16772146487226855No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT59340.16366702393554375No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG53790.148359440807093No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT53290.14698037926398932No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG49580.1367477426141601No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG46320.12775626135312415No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44610.1230398708757096No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG44550.12287438349053717No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT43130.11895784870812273No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA40530.11178672868398365No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG37040.10216087911312001No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA36700.10122311726380952No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG36500.10067149264656804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15350.042.5894570
CGCGGCA49900.037.96544332
GCGGCAC50750.037.33473633
CGGCACC51550.036.56097434
TCGCCGG52100.034.37431748
CCGTCGC50900.034.31608245
CCCGTCG51500.033.91204544
CCCCCGA57150.033.2604619
ATGGCCG15550.033.2073969
ACCCGCG57000.033.12833425
GCACCCC57350.032.9954436
GGCACCC58250.032.42154735
CCCCCGT54050.032.29734442
CACCCCC59750.031.79204437
CCCCGAC61450.030.87797220
GTCGGTT2750.030.206114
GTTCCGT3450.030.2029531
CGCCCTA10450.029.87990261
CCGCGCG64800.029.1475127
CGCGAGA6250.028.82712425
Produced by FastQC (version 0.11.5)