FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439784
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG176350.5126775402176416No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA153060.44496980042932927No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC136260.39612952441199795No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135470.39383286857546873No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC114200.3319975905463831No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT72290.21015854483886195No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG58190.16916759889574462No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA41950.121955332078991No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40310.11718759084872771No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37290.10840796980275506No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG36360.10570431166608135No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG35660.10366930016535922No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT35620.10355301379388938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG38600.063.215024
TCTACGG38800.062.8014565
TACGGCC39150.062.067977
CTACGGC39650.061.4551436
ACGGCCA41450.058.7060178
GGGTCTA30950.056.843432
GCGTCTA7500.056.2614332
CGGCCAT43300.056.119199
GCCATAC43750.055.61001611
CCTGGTT62500.055.3758670
CCATACC44400.054.80150612
CATACCA44700.054.58596813
GGCCATA44800.054.468110
GCGCGGT65000.053.143949
CGTCTAC9550.052.3798643
TACCACC47400.051.40259615
CGCGGTG68300.050.62605310
GCCGGTT10750.050.0148543
CCGAACT10650.050.01281467
CGGTCTA5600.049.8283162
Produced by FastQC (version 0.11.5)