FastQCFastQC Report
Wed 8 May 2024
EGAF00005318784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391346
Total Bases250.7 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG273810.8073785452737644No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC239850.7072413136259172No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC218060.6429895386669482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130790.38565808384045747No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG100380.295988672344255No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT97450.28734903486698204No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG89160.26290446330159173No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGT83880.24733542375210316No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT80200.23648427497518684No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCT64920.1914284180971213No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA62930.18556054144873452No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT60260.1776875612220045No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC59480.1753875894703755No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC54930.16197108758587297No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA54110.15955316856492968No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT53700.15834420905445803No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG49580.14619564031508434No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG45420.13392912430639634No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG44730.13189453391072453No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT42930.1265869067915807No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG42620.12567281545439482No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC42310.12475872411720891No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC41530.12245875236557992No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG40620.11977545198867943No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39580.11670882298650742No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTTGGGGGAGGCCCAAGGGGCAAGAAG39120.11535242938939289No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG38720.11417295669624981No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA38410.11325886535906392No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA36170.10665381827746269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.1061525423828769No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35890.10582818739226253No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCC34760.10249617703413334No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG34750.10246669021680478No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA34530.10181798023557607No Hit
ACTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCG34510.10175900660091892No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG34400.10143465161030459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)