FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363457
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG166330.4945209645908956No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA143040.4252767316484201No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131560.39114518187686065No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC130610.3883207069393187No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC109250.32481461781732307No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT70090.20838678776033112No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG57560.17113344989990953No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC39210.11657648663265206No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA38590.1147331450944668No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC35720.10620025765157694No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34270.10188921695743398No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG34060.10126485933966153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG37750.063.6666155
GTCTACG38250.062.9119764
CTACGGC38800.062.0312926
TACGGCC38850.061.947767
CGTCTAC9800.058.9595763
CCGAACT12000.058.77189367
ACGGCCA41150.058.650528
CGGCCAT41150.058.4870579
GCCATAC41200.058.25192611
GCGTCTA8850.057.627352
GGCCATA42600.056.416510
CCATACC43350.055.51387812
CATACCA43400.055.4499213
GCCGGTT12400.054.82013
GGGTCTA30550.054.7563552
CCTGGTT59850.054.2805670
CGGTTCA12750.053.591415
TACCACC44950.053.3866215
GCGCGGT66300.051.887579
CCGGTTC13300.051.621574
Produced by FastQC (version 0.11.5)