FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291705
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA134290.4079648692698769No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC111120.3375758155727807No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC88540.2689791460656408No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84230.255885627661045No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63300.19230155800717258No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG51160.15542097484434358No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46350.14080848678724248No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT33660.10225703700665766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG17650.059.809625
TACGGCC17750.059.4726647
GTCTACG18250.057.463974
CTACGGC18550.057.0943876
GCCATAC19250.054.46889511
CCGAACT9450.053.54093667
TCGCGTA200.006884868352.6312360
GCGCGGT49000.052.2698869
CGGCCAT20450.051.959029
CGGTTCA9800.051.5635345
ACGGCCA20750.051.3746078
TGGCGCG51150.049.935215
CCATACC21300.049.5582912
CATACCA21350.049.1180213
GCCGGTT10400.048.58653
CGCGGTG53350.048.46208210
CGAACTT10800.048.24127668
CCGGTTC10600.047.6719474
GGCCATA22250.047.4444810
ATCTCGT22400.046.97199235
Produced by FastQC (version 0.11.5)