FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277199
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA133210.4064751636992444No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC112030.341846802711706No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC87620.2673624641042549No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84430.25762854193474366No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61880.18881978177095746No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50160.15305753480334883No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45940.1401806847860017No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT34310.10469306258179623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG16250.059.859325
TACGGCC16350.059.4904867
CTACGGC17450.055.7403686
GTCTACG17450.055.544554
GCGCGGT50300.053.8798269
CCGAACT9900.052.88916467
TGGCGCG52700.051.42531215
ACGGCCA19000.051.372178
CGCGGTG53350.050.9292910
CGGCCAT19100.050.9219939
GCCATAC19200.050.29622711
GCCGGTT10800.049.68063
CGGTTCA10900.049.224815
CGAACTT11050.048.76583568
CATACCA20350.047.28312313
GAACGCG20550.046.70944224
GGGTCTA16250.046.6690372
GGCCATA21400.045.61080610
ATCTCGT21400.045.10021235
TGAACGC21300.045.06130223