FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284400
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA128840.3922786505906711No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC109970.3348252344416027No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89630.2728961149677262No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84650.25773352819388623No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63190.1923943490439654No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50680.15430520034100595No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46770.14240043843624406No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC33480.1019364267446109No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT33250.10123614663256607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG16800.062.4283375
TACGGCC17300.060.222987
GTCTACG17900.058.978714
CTACGGC18500.056.6890956
GCCATAC19250.054.30227711
ACGGCCA19450.053.74398
CCGAACT9650.053.51532767
GCGCGGT49300.053.0782669
CGCGGTG51500.050.94526310
GCGTCTA4150.050.878792
CATACCA20950.050.5567613
CCATACC20750.050.5436212
CGGCCAT20850.049.9691859
GCCGGTT10250.049.6419143
CGGTTCA10400.049.590835
TGGCGCG52950.049.2871815
GGGTCTA16550.048.1044122
GGCCATA22200.047.39826610
CGGCATA1850.047.0909961
CCGGTTC11000.046.5712054