FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287305
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA125280.3811024532253624No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC107370.326620134122024No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC86100.2619166764264344No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG80880.24603740754204434No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT60880.185197296873883No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG47900.1457120650502463No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45160.13737696988870823No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT32970.10029492243646393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG18050.059.620125
TACGGCC18200.059.5097
CTACGGC18700.058.102896
GTCTACG19400.055.999574
GCGCGGT53000.053.9279449
GCCATAC20600.051.73604211
CGGCCAT21050.050.9588139
ACGGCCA21300.050.6856088
GGGTCTA16800.050.6782382
CGCGGTG56550.050.66492510
CCGAACT9050.050.0544367
TGGCGCG56900.049.679815
CCATACC22100.048.22014212
CGGTTCA9550.047.827785
GGCCATA22850.047.55028510
CGGATAT2200.045.9929281
CCGGTTC9950.045.8994644
CATACCA23150.045.88358713
GCCGGTT10200.045.1136783
GAACGCG23650.045.10997424