FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158732
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG197450.4747841409352659No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC179750.4322230910767994No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC151700.3647746476570262No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG89560.21535410312566428No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG83050.19970029326246558No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT76130.1830606059731668No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG65320.15706710603135765No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG63870.15358046635368666No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG60300.1449961190093519No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT55940.13451215418545845No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG53240.12801979064772628No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT48250.116020940998362No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC46920.1128228508112569No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45280.10887934110685661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT20750.042.67327570
CGCGGCA60200.037.34023332
AGGACCG7400.036.71330670
GCGGCAC62350.036.23153733
CGGCACC64000.035.3093834
ACGATCG501.8522983E-434.5314876
CCGTCGC62400.034.3699845
TCGCCGG62600.034.153448
CCCGTCG64450.033.4217944
GCACCCC69400.032.73234636
CTTACCC16300.032.7174770
CCCCCGA70650.032.51718519
GTTCCGT4400.032.206911
ATGGCCG22050.031.90230669
CACCCCC72800.031.40595437
GGCACCC72100.031.34939835
TCAGGAT28250.031.16599870
ACCCGCG73900.030.69411325
TAGTCCG3650.030.274186
GTCCGTT2850.029.0885962