FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284106
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG201580.4705299075232966No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC186700.4357968733733479No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC158820.3707191185278796No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG93460.21815519970794373No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG87180.20349636540272345No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT77520.18094790371666808No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG67730.1580959948236575No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG63880.1491092890792151No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG60290.14072947774868316No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT57420.13403029710282613No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54040.126140669721991No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT50290.11738738490597572No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC48300.11274230843027694No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45800.10690678521960008No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG43460.10144473549440654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT22700.045.5128270
ACGATCG459.958504E-538.3735776
CGCGGCA59950.036.98167832
GTTCCGT3850.036.830031
GCGGCAC62100.035.60226433
ATGGCCG23100.035.4592869
CGGCACC63550.034.6377234
CCGTCGC62100.034.305845
TCGCCGG63250.033.46045748
CCCGTCG64550.033.31159644
TCAGGAT30800.032.72937870
GCACCCC68850.032.34350636
CTTACCC16250.031.17169670
CCCCCGA72300.031.0166119
GGCACCC72000.030.9186835
CACCCCC73550.030.33380937
ACCCGCG76450.029.54477325
GTCGGTT4100.029.4862734
TATGGCC26500.028.61970968
AGGACCG7950.028.38819770
Produced by FastQC (version 0.11.5)