FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284106
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG196930.4596758343514376No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC161210.37629787871728665No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC135810.31700896289680974No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA88870.20744117909314103No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT79230.18493940159277103No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG55510.12957195736986898No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48600.11344257121555816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG42150.062.421145
GTCTACG42550.061.752474
TACGGCC42700.061.385297
CTACGGC43350.060.617826
ACGGCCA46450.056.801278
CGGCCAT46450.056.3551869
GCCATAC47450.055.2396511
CCTGGTT67850.054.13894370
GGGTCTA38000.053.9718132
GCGCGGT78850.052.863519
CCATACC50150.052.2650212
CATACCA50550.051.98686213
GCCGGTT13350.050.6919443
GGCCATA51600.050.59673710
CGCGGTG83050.050.19009810
GAACGCG52100.050.16626424
CTGAACG52600.050.07145322
TACCACC52200.050.07137715
GCGTCTA8700.050.0052342
TGAACGC52900.049.72971723