FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360411
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG236740.7044971582345135No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC105530.314038967257279No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC89140.2652651714328991No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG62820.18694141877288226No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA60120.17890668730699907No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC57460.1709909888998697No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47470.14126248247610187No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG43050.1281093294838042No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37050.11025437067073045No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34220.10183278176389733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG46600.063.7116935
GTCTACG46750.063.433514
TACGGCC47450.062.570397
CTACGGC48200.061.5967836
CCTGGTT69850.060.01957770
ACGGCCA49900.059.6365058
GCGTCTA9700.058.6675872
CGGCCAT51250.057.9983069
GCCATAC51500.057.6463711
GGGTCTA37150.057.2812352
CCATACC52550.056.56015412
CATACCA53300.055.6995913
GGCCATA53650.055.40048610
CGTCTAC11750.054.8789333
TACCACC54850.053.87653415
CCGAACT8400.053.65496467
CGGTCTA5550.053.443082
GAACGCG55350.053.1511824
CTGAACG56250.052.65337422
TGAACGC56200.052.58286323