FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454591
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG239820.6942066369072345No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103840.30058551070155626No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC90100.2608123508687425No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG65380.18925539955381115No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA63250.18308969137012168No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC56710.16415836201738498No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT48220.13958237024296075No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG44130.12774305265080585No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC36740.10635122942194894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG46600.063.7132455
GTCTACG46650.063.6449554
TACGGCC46850.062.943497
CTACGGC48000.061.5711486
CCTGGTT68550.060.067570
ACGGCCA50450.058.588718
GGGTCTA36050.058.54082
GCCATAC50300.058.35201611
CGGCCAT50600.058.2787069
GGCCATA51600.057.21611410
GCGTCTA9650.057.1748622
CCATACC52500.055.97248512
CATACCA53200.055.42969513
TACCACC54500.054.03405415
CGTCTAC11100.053.7446943
CGGTCTA6350.053.218772
CTGAACG56250.052.27280422
GAACGCG56400.052.20935424
TGAACGC56550.052.06482323
GCGCGGT52900.051.5069359