FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413135
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG133150.3901105581818475No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC95050.27848297825899065No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC75890.2223469039460789No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG67550.1979118903881622No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA62520.1831747059521525No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG60210.1764067345710029No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG48490.14206880184932622No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT39780.11654974092732927No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36830.10790666059209496No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35230.10321888820688313No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC34780.10190045222354228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT21100.047.425870
TAGTCCG2350.039.640146
CGCGGCA45850.036.6925632
CGTACGT1700.036.5504119
AGGACCG7550.035.96589770
GCGGCAC46650.035.77544833
CGGCACC48550.034.4553234
ATGGCCG23100.034.33310769
GTGCCGT4150.034.1430051
CCGTCGC46750.033.7952345
CCCGTCG46800.033.66534444
TCGCCGG47250.033.4242848
TATCGGA1450.033.3118978
GACGTAT4400.031.61826544
GCACCCC54500.030.97158436
CACCCCC55400.030.54404837
CCCCCGA56950.030.12529819
CGCCCTA25100.029.956161
GGCACCC56700.029.76065835
TTATCGG1750.029.5728057