FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934315
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG107050.364821091123482No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC104770.35705096419436905No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC70310.23961299315172366No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG40520.13809015051212975No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT38520.1312742496971184No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG30340.10339721536372204No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC29750.10138652462329369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA32450.036.4772432
GCGGCAC33850.034.9715733
TCGCCGG33250.034.15088748
CGGCACC34850.034.07118634
CCCGTCG33250.034.01231444
CCGTCGC33200.033.9674545
CGTTGGA5450.032.48576433
TTCGTTG5350.032.4352331
ACCCGCG38300.031.5983325
CCCCCGA38250.031.43739519
GCACCCC39950.030.25292636
GATTTCG4150.030.09021228
CCCCGAC42400.028.44454220
AACGGGA4650.028.40141740
GATAGGC31700.028.06661640
GGCACCC42400.028.00953135
AGTCCGC44050.027.9231437
CACCCCC43800.027.5200637
CAGTCCG43350.027.255066
AAACGGG5900.027.09291639