FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988934
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG113880.38100540192590404No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC103000.3446044643341071No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC70500.23587004597625774No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG42830.14329523502359034No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT39010.1305147587735293No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC32030.10716195138467427No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG30570.10227726674459858No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT30230.10113973744485492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA6250.044.99089433
TTCGTTG6900.042.25276631
TCGTTGG7050.040.37592732
GATTTCG4850.040.05463828
CCGCGTA350.001318176239.6094062
CGCGGCA32600.036.52366332
AAACGGG6200.036.43407439
GCGGCAC33500.035.4405933
AACGGGA5850.035.05306240
ATTTCGT5600.034.69482829
CGGCACC34600.034.31904634
CCCGTCG33600.034.26584644
GAAACGG6750.033.9739538
TCGCCGG33850.033.84878548
CCGTCGC33850.033.8174945
GGAAACG6600.033.16226637
CTAGACG1400.032.55220864
CGGGAAT4300.031.53453642
CGTACGT553.173243E-431.50537319
CCCCCGA38450.031.27594219