FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988934
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA72140.24135695201031537No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC59720.19980367582556188No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54190.18130209633267244No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC50160.16781902845629915No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC33380.11167861183953877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC11050.057.033257
TCTACGG11400.055.889725
CTACGGC11400.055.2822276
GTCTACG11700.054.4566574
CGGTATA2750.053.0389821
ACGGCCA13400.047.5479748
TGGCGCG30500.046.54899215
GAACGCG13950.045.70162624
GCGCGGT31050.045.5006459
CGGCCAT14250.044.4687849
GCGTACT2900.044.3080181
ATCTCGT14150.044.14941835
CCCGATC14450.043.9290431
CGGTATC2400.043.409881
GCCCGAT14700.043.40554430
CTGAACG14550.043.33059722
CGGTACT2900.043.1105041
TGAACGC14800.042.60293623
CGCGGTG33450.042.5458710
CGCCCGA15200.041.972129
Produced by FastQC (version 0.11.5)