FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085567
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG114470.37098530027058235No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC105760.3427571010449619No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC72150.23383060552566187No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45000.14584029450664981No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT39140.12684864726645054No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC33160.10746809257423352No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG31850.10322251955637327No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT31320.10150484497662828No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG31230.10121316438761498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA30350.040.13958732
GCGGCAC31550.038.39723233
CGGCACC32750.037.20591434
CCCGTCG32050.034.95408644
CCGTCGC32050.034.746645
TCGCCGG32200.034.7216748
CCCCCGA36450.034.2201619
TTCGTTG7350.033.99595631
TCGTTGG7450.033.54290832
TGGCCGT8650.033.32198770
CGTTGGA7400.033.30431433
GCACCCC38350.032.23868636
ACCCGCG38150.032.09076725
TTTCGCG553.0965128E-431.63717845
CCCCGAC40600.030.55458320
CACCCCC40800.030.396837
TAGTCCG2850.030.37966
GATTTCG5500.030.28435528
AAACGGG7200.029.92550739
GGCACCC41150.029.87285435