FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049082
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG112800.36994741368057665No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC103600.3397743976711679No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC71200.233512906507598No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG44450.14578158278458894No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT37990.12459487806493888No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG31900.10462165333697158No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC31460.10317859604956509No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT31040.1018011322752225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA33200.038.38220632
GCGGCAC34550.037.0883433
TCGTTGG6400.036.89188832
CGTTGGA6500.036.329133
CGGCACC35300.036.20494534
TTCGTTG6950.035.46417231
CCGTCGC34000.034.91863345
TCGCCGG34100.034.63658548
GATTTCG5350.034.37692628
CCCGTCG34400.034.19882244
ACCCGCG40000.032.258925
AAACGGG6700.032.1713339
CCCCCGA39900.032.14317719
GCACCCC40600.031.91688236
GATAGGC31550.030.74780840
GGCACCC43000.030.28958535
AACGGGA6000.030.13432140
CCCCGAC43450.029.68042620
ATTTCGT6350.029.51262329
GAAACGG7450.029.39200238