FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049082
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA74210.2433847302237198No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC60090.1970757100005838No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54650.17923427444719428No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC50450.16545963670376856No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC32910.10793412574670017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG11500.059.9130364
TCTACGG11750.058.344585
TACGGCC11850.057.560987
CTACGGC12100.056.657866
CGGTATA2950.055.3566441
ACGGCCA13650.049.7168668
GAACGCG13850.048.79218324
GCGTATA3350.048.74691
CGGTATG3000.047.4850121
CGGCCAT14300.047.214119
CCCGATC14700.046.96245231
GCGCGGT31800.046.7093059
TGGCGCG32300.045.98473715
GCCCGAT15250.045.26276830
TGAACGC15150.044.83045623
ATCTCGT15300.044.68641335
CGCGGTG33500.044.3389810
TCTCGGA15700.044.33724646
CGGTATC2450.043.9632261
CGCCCGA15700.043.95747829
Produced by FastQC (version 0.11.5)