FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285502
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC152760.4649517790584209No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149370.45463372111780787No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG147100.4477245790749785No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC122010.3713587756148071No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT61370.186790329149092No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT59010.17960725636447641No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT54000.16435844507171204No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG53990.16432800832262467No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50200.15279248041851748No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG49150.1495966217643453No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG47540.1446963051612813No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA46230.1407090910308379No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTTGGGGGAGGCCCAAGGGGCAAGAAG44580.13568702743142447No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41870.12743866842875154No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC40710.12390800553461845No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG39480.1201642853968739No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT39440.12004253840052449No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.11885550518611768No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG36110.10990710095443558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT24050.048.61652870
TATCGGA2300.038.4596828
TTATCGG2400.038.274787
GTTATCG2350.037.641396
CGCGGCA45950.037.30806432
GCGGCAC46850.036.47352233
TCAGGAT25100.036.39278870
CGGCACC47650.035.52649734
ATGGCCG25800.035.21363469
CCGTCGC47700.034.19169645
TCGCCGG48400.033.8302448
CCCGTCG48200.033.7272644
GCACCCC53650.032.0048336
AGGACCG7200.031.8985770
CCCCCGA54000.031.70011519
CCCCCGT51550.031.45342842
GGCACCC54250.031.30257235
ACCCGCG55150.031.17144225
ATCGGAA3000.030.6198279
CTTACCC15800.030.06320470
Produced by FastQC (version 0.11.5)