FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179932
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG116930.36771226554530095No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC82740.26019424314733774No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA75850.2385271131583946No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC72670.22852689931734388No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48050.15110386008254265No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45090.14179548493489796No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG32310.10160594629067539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG25000.061.5853774
TACGGCC25800.059.9393277
TCTACGG25650.059.8907475
CTACGGC26450.058.0783966
GCCATAC29000.053.44383611
ACGGCCA29500.053.2369588
CGGCCAT29600.052.7088059
GCGTCTA5700.051.8721472
CCATACC30250.051.23541312
GCGCGGT42550.050.8815169
GGCCATA31300.050.17540710
CCTGGTT43850.050.07155670
GGGTCTA22400.049.8822482
CATACCA31200.049.78550313
GAACGCG30900.049.70804224
ATCTCGT31450.048.9789435
TGGCGCG44650.048.33450715
TGAACGC32150.047.8666423
CCCGATC32350.047.52282731
TACCACC33550.046.19586615