FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187343
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG117760.3694613350367375No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC84270.26438949306679577No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA77370.24274136796698692No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC71490.2242934004906281No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47990.15056427877388784No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT44740.1403676981109344No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34020.10673466897036182No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC31900.10008336096868142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG25050.062.0350154
TCTACGG25150.061.7883535
TACGGCC25600.060.835577
CTACGGC26800.057.98246
CGGCCAT28600.054.3340079
GCCATAC28750.053.57304411
ACGGCCA29150.053.544728
CCATACC29800.051.80077712
GCGCGGT40350.051.2923169
GGGTCTA22750.050.6263242
CCTGGTT44400.050.1044470
CATACCA30900.049.9567313
GAACGCG31200.049.14216224
TACCACC31600.048.95812615
TGAACGC31600.048.50559623
ATCTCGT32000.048.47016535
TGGCGCG43100.047.9398415
GGCCATA32550.047.846110
CCCGATC32400.047.57074431
CCGAACT7650.047.34711567