FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166547
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA167310.5283673351445597No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC134750.4255423968126795No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG131000.41369984402568477No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC118830.37526681271429096No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT59860.18903872262120222No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC59850.18900714248043687No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA47610.1503530501836859No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC44660.14103690865791665No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC39930.12609950207592055No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA36930.1166254598463247No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35930.11346744576979277No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG31990.10102487030825692No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC31940.10086696960443031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC26350.062.6889847
TCTACGG26450.062.0637785
GTCTACG26500.061.9466744
CTACGGC27700.059.512076
ACGGCCA28350.058.630488
GCCATAC28700.057.3146911
CGGCCAT29050.057.0989889
GGGTCTA22150.055.134542
GGCCATA30150.055.1301510
CCATACC30150.054.44388212
CATACCA30150.054.32950213
CCTGGTT42800.053.25105770
CGGTCTA3450.052.996962
GCGCGGT62450.052.569529
TACCACC31700.051.8905915
GAACGCG32150.050.91974324
CTGAACG32350.050.8088122
TGAACGC32750.050.3000523
CGCGGTG65750.050.14084210
GCGTCTA5650.050.067932
Produced by FastQC (version 0.11.5)