FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224507
Sequences flagged as poor quality0
Sequence length35-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA124290.3854542725446092No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG115520.3582563163919322No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC115010.35667467926104673No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC98970.30693064087006167No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT55590.17239844726651238No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC51510.1597453502194289No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46100.1429675916349383No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41940.13006639464575515No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA38240.11859177232364512No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG34190.10603171275484903No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC32760.10159692628981733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23400.060.6014984
TCTACGG23850.059.602395
TACGGCC24200.058.740377
CTACGGC24950.057.388496
GGGTCTA19450.055.7398722
GCCATAC25750.054.52943411
ACGGCCA26250.054.153038
CATACCA26950.052.3608713
CGGCCAT27150.052.35799
GCGCGGT57950.051.9704679
CCATACC27450.051.4071212
GGCCATA27850.050.9183210
GCGTCTA4600.050.129342
CGCGGTG60500.049.77997610
CCGAACT8200.049.7473167
TGGCGCG61450.049.62267315
TGAACGC28650.049.3628123
CCTGGTT37900.049.27590670
GAACGCG28850.048.9155124
CGGTTCA8450.048.0648165
Produced by FastQC (version 0.11.5)