FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201088
Sequences flagged as poor quality0
Sequence length35-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA125130.3908983445628486No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG115500.3608148229601935No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC112770.3522864725993162No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC96320.3008976947837735No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT56750.17728347361896954No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC54370.16984850150948677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46910.14654392506547773No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT42140.13164274146790092No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA37600.11746006357838336No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG35240.11008757022612312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21650.061.8882454
TCTACGG21700.061.4281855
TACGGCC22250.060.0626567
CTACGGC23050.057.8286256
GCCATAC24050.055.27916311
CGGCCAT24350.054.8809979
GGCCATA24850.053.77675210
ACGGCCA25000.053.729638
CATACCA25100.053.1039113
CCATACC25050.053.0724112
CCGAACT8050.052.839367
GGGTCTA19100.051.226722
CGGTTCA8350.051.1507075
GCGCGGT54400.050.9666679
GCCGGTT8650.048.5802963
CGCGGTG57750.048.1294410
TACCACC28450.046.97196615
GCGTCTA4850.046.882822
CCTGGTT37450.046.848570
TGGCGCG59300.046.81333515