FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215397
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA114970.3575608237489803No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG113280.3523048631319865No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC108840.3384963038778726No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC94040.2924677730308264No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT53000.1648319010063143No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC52010.1617529654969511No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46740.14536307647236096No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT39790.12374832718945747No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA37340.1161287393127505No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG33060.10281778579752361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG24700.060.5077975
TACGGCC24900.060.021797
GTCTACG25050.059.7951854
CTACGGC25800.057.7945336
CGGCCAT27250.054.971979
GCCATAC27000.054.71571711
CATACCA27100.054.2588213
GGGTCTA21100.053.7039762
CGCGTAA607.6397555E-1153.0998961
ACGGCCA28300.052.932378
CCATACC27850.052.92128412
GGCCATA28500.052.44008610
GCGCGGT53900.051.2394149
CCTGGTT39550.049.61445270
CCGAACT8650.049.5880867
TACCACC30200.049.0305215
CGGTTCA8650.048.9674575
TCGCGTA651.6734703E-1048.95958360
GCGTCTA5600.048.5883452
GTCGTAC1350.048.4623573
Produced by FastQC (version 0.11.5)