FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15519093
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA54050.037.7175133
GATTTCG38850.037.61738628
TCGTTGG54450.037.37303532
TTCGTTG57450.036.38564331
CGGGAAT36150.034.62331842
AAACGGG51750.034.2482239
CGAAACG5200.032.73913237
GAAACGG57100.032.6184538
TTACTCG14550.032.1101657
AACGGGA44800.031.96415740
ATTTCGT45800.031.91205229
TCGTTCG4000.030.38551732
CGCGGCA25100.029.46893932
ACGGGAA32150.028.87012541
GGAAACG59200.028.69865637
GTGGATA93750.028.1968925
ACCCGCG29400.026.0886425
TTTCGTT64600.025.90591230
TCGCCGG25900.025.53032348
AACGGGT12900.025.05747240
Produced by FastQC (version 0.11.5)