FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15457535
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG52050.036.5665532
CGTTGGA53900.035.24933633
GATTTCG39000.035.07209428
TTCGTTG57300.033.63669631
AAACGGG52300.032.1685139
CGGGAAT32550.031.72753742
AACGGGA42950.031.486440
GAAACGG58550.030.39322938
TTACTCG14700.030.35403857
ATTTCGT46550.029.8360129
CGCGGCA25750.029.13403932
GGAAACG58500.027.5590137
GTGGATA91150.027.1366675
CGAAACG6400.026.60220337
CCGTCGC26550.026.19979345
TCGCCGG26800.026.09659648
ACCCGCG29200.025.91259825
AACGGGT12950.025.77140240
CCCGTCG27300.025.60891244
CCGCGCG30750.024.83579827
Produced by FastQC (version 0.11.5)