FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15457535
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA28300.050.866891
GCGTATT29950.049.228331
GCGTACT22400.047.7707331
CGGTATT36100.047.0211561
CGGTACT23550.046.7700351
GCGTACA27600.046.600311
GCGTATA26650.045.776481
CGGTATG23200.045.0717931
CGGTACA31500.045.0355451
GTCTACG19100.044.251854
CGGTATC18850.043.4538271
GCGTAAT20500.043.3568651
GCGTACC14750.042.7718541
TACGGCC19800.042.6864247
CGGTGTA24200.042.4891661
CGGTAAT28250.042.196771
CGGTACC20400.041.6899341
GAACGCG19850.041.37638524
GGGTATT123750.041.037971
GCGTATC17100.040.766691
Produced by FastQC (version 0.11.5)