FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020319
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG131990.4370068194783399No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA115940.38386673725523696No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC96650.31999931133102166No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC82610.27351415529286804No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45940.15210313877441423No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45890.1519375933469279No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38510.12750308824994977No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC33790.11187559989524284No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC31850.10545243730877435No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA31250.10346589217893871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG27050.061.2359735
TACGGCC27050.060.7246677
GTCTACG27850.059.6008644
CTACGGC28400.058.325116
ACGGCCA29050.056.781558
GCCATAC29650.055.17337411
GCGTCTA5950.054.534732
GGGTCTA24000.053.217292
CCATACC31050.053.1320512
GGCCATA31250.052.67806610
CGGCCAT31450.052.5590559
CCTGGTT44450.051.69344770
CATACCA31850.051.68912513
GCGCGGT47500.051.1460049
CCGAACT7950.050.64716367
GCCGGTT8200.050.0893023
GAACGCG33900.048.52995324
ATCTCGT34100.048.2689635
TACCACC34400.048.25884615
CGCGGTG50600.048.08394610