FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016305
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG162900.5400647480941085No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC140540.46593431367186006No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC118630.3932957708189324No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG69680.23101112122282064No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG66120.21920860125219432No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT57310.1900006796394927No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG53420.1771041058513645No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG49710.1648042886909646No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG48320.16019600139906276No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG45630.1512778051291232No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT43620.14461402278615723No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC39860.1321484398958328No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC39560.13115384551628564No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT36260.12021330734126687No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35590.11799204656027822No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG34390.11401366904208957No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA31340.10390195951669344No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC31220.10350412176487458No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC30780.10204538334153875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT13450.036.64155270
CGCGGCA54800.034.97014632
GCGGCAC55400.034.47261433
CGGCACC57500.033.34305634
CCCGTCG56750.032.07002644
CCGTCGC57050.031.73218245
TCGCCGG57250.031.73248
AGGACCG7100.031.55114270
CGTACGT1650.031.42307719
GCACCCC62500.030.7556636
CCCCCGA63350.030.60959419
ATGGCCG13000.030.04419369
CACCCCC64050.030.0183837
GGCACCC64500.029.79057935
TCAGGAT19900.029.26804770
TAGTCCG2600.029.2345286
GTCCGTT2250.029.18372
GGTAGAC9100.028.99315370
ACCCGCG66650.028.91635525
GTTCCGT3000.028.8236221
Produced by FastQC (version 0.11.5)